Purified recombinant Hsp70 was electrophoresed in SDS-PAGE, transferred to nitrocellulose membranes and cut into 2 – 3 mm wide strips. These were incubated with the plasma and the presence of antibodies to Hsp70 detected as described in Methods. Lane 1 – 3: positive; Lane 4: negative.
Yang et al. Respiratory Research 2005 6:18 doi:10.1186/1465-9921-6-18