A. Lymphocyte proliferation following stimulation with Ppg and Derf1 was increased in CBMC (p < 0.001). B. IFN-γ secretion was increased following stimulation with Ppg as compared to unstimulated CBMC (U) (p < 0.001). C. IL-13 secretion was increased following Ppg stimulation as compared to unstimulated cells (U)(p = 0.001). D. TNF-α production was increased following stimulation with either Ppg or Der f 1 compared to U (p < 0.001). E. IL-10 production was increased following stimulation with Ppg as compared to U (p < 0.001). A-E. Lymphocyte proliferation and cytokine concentrations from supernatants of CBMC were determined following stimulation with the indicated doses of Ppg and Derf1. Lymphocyte proliferation shown as SI (stimulation index, ratio of mean counts per minute of stimulated over unstimulated replicates) was measured by 3H-Thymidine uptake, cytokine concentrations were measured with ELISA (Methods)(n = 50). Data are shown as Box- and whiskers- plots (Median, whiskers: 5% and 95%-quantile) with outliers.
Schaub et al. Respiratory Research 2006 7:40 doi:10.1186/1465-9921-7-40